PRODUCTS

  • A general protocol for creating high-throughput screening

    30-06-2009· A general approach to high-throughput screening of enantiomeric excess (ee) and concentration was developed by using indicator displacement assays (IDAs), and the protocol was then applied to the vicinal diol hydrobenzoin. The method involves the sequential utilization of what we define herein as screening, training, and analysis plates.

    A cell-based high-throughput protocol to screen entry

    Previously we reported a high-throughput screening (HTS) protocol to identify entry inhibitors for three highly pathogenic viruses (EBOV, LASV, and AIV) using a human immunodeficiency virus-based pseudotyping platform which allows us to perform the screening in a BSL-2 facility.

    High Throughput Screening Methods and Protocols

    In High Throughput Screening: Methods and Protocols, Second Edition, experts in the field fully update the previous edition to best serve as an introduction to HTS for scientists who are just entering the field, and as a provider of key, significant detail so as to be useful to scientists in established HTS

    A High-Throughput Screening Protocol for Fast Evaluation

    A new high-throughput screening protocol that allows fast evaluation of enantioselective catalysts has been developed. The usefulness of norephedrine-derived β-amino alcohols as catalysts for the enantioselective alkylation of prochiral aldehydes has been determined by simultaneous screening of three representative substrates.

    Cell-Based High-Throughput Screening Protocol for

    19-01-2021· In this study, we developed a protocol for high-throughput screening (HTS) to identify inhibitors against SARS-CoV-2 proteases based on the split-GFP complementation method. Our previous published data showed a practical implementation of split-GFP complementation assay to measure protein translocation from ER-to-cytosol .

    A High-Throughput Screening Protocol for Fast

    A new high-throughput screening protocol that allows fast evaluation of enantioselective catalysts has been developed. The usefulness of norephedrine-derived β-amino alcohols as catalysts for the enantioselective alkylation of prochiral aldehydes has been determined by simultaneous screening of three representative substrates.

    A general protocol for creating high-throughput

    30-06-2009· A general approach to high-throughput screening of enantiomeric excess (ee) and concentration was developed by using indicator displacement assays (IDAs), and the protocol was then applied to the vicinal diol hydrobenzoin.The method involves the sequential utilization of what we define herein as screening, training, and analysis plates.

    Cell-Based High-Throughput Screening Protocol for

    19-01-2021· Thus, developing novel screening methods and direct-targeting viral enzymes could be an attractive strategy to combat SARS-CoV-2 infection. In this study, we designed, optimized, and validated a cell-based assay protocol for high-throughput screening (HTS) antiviral drug inhibitors against main viral protease (3CLpro).

    A multifunctional cross-validation high-throughput

    The protein tyrosine phosphatase Src homology phosphotyrosyl phosphatase 2 (SHP2) is implicated in various cancers, and targeting SHP2 has become a promising therapeutic approach. We herein described a robust cross-validation high-throughput screening protocol that combined the fluorescence-based en

    A comparative high-throughput screening protocol to

    One of the challenges of developing antivirals is that most of these viruses are highly pathogenic and therefore require high biosafety-level containment. In this study, we have adopted a comparative high-throughput screening protocol to identify entry inhibitors for three enveloped viruses (Marburg virus, influenza virus H5N1, and Lassa virus) using a human immunodeficiency virus-based

    A modified high-throughput screening protocol to isolate

    19-02-2021· A modified high-throughput screening protocol to isolate bacteriophages from environmental samples Abdallah S. Abdelsattar 1,2, 1Rana Nofal1, Salsabil Makky,Amera El-Sayed1, Ayman El- Shibiny1,* 1 Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza, 12578, Egypt

    High Throughput Screening: Methods and Protocols

    High Throughput Screening: Methods and Protocols; find Sigma-Aldrich-H8161 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich

    High-throughput screening (HTS) BMG LABTECH

    10-04-2019· High-throughput screening in drug discovery. The primary goal of HTS is to identify through compound library screenings, candidates that affect the target in the desired way, so called “hits” or “leads”. This is usually achieved employing liquid handling devices, robotics, plate readers as detectors and dedicated software for instrumentation control and data processing.

    High Throughput Screening an overview ScienceDirect

    High throughput screening (HTS) is the use of automated equipment to rapidly test thousands to millions of samples for biological activity at the model organism, cellular, pathway, or molecular level. In its most common form, HTS is an experimental process in which 103 –10 6 small molecule compounds of known structure are screened in parallel.

    Data Analysis Approaches in High Throughput Screening

    aspects of high throughput screening data interpretation. 2. Role of statistics in HT screening design 2.1. HT screening process A typical HT screening campaign can be divided into five major steps regardless of the as‐ say type and the assay read-out (Fig. 1).

    A High-Throughput Screening Protocol for Fast

    A new high-throughput screening protocol that allows fast evaluation of enantioselective catalysts has been developed. The usefulness of norephedrine-derived β-amino alcohols as catalysts for the enantioselective alkylation of prochiral aldehydes has been determined by simultaneous screening of three representative substrates.

    A general protocol for creating high-throughput

    30-06-2009· A general approach to high-throughput screening of enantiomeric excess (ee) and concentration was developed by using indicator displacement assays (IDAs), and the protocol was then applied to the vicinal diol hydrobenzoin.The method involves the sequential utilization of what we define herein as screening, training, and analysis plates.

    A modified high-throughput screening protocol to isolate

    19-02-2021· A modified high-throughput screening protocol to isolate bacteriophages from environmental samples Abdallah S. Abdelsattar 1,2, 1Rana Nofal1, Salsabil Makky,Amera El-Sayed1, Ayman El- Shibiny1,* 1 Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza, 12578, Egypt

    High Throughput Screening: Methods and Protocols

    High Throughput Screening: Methods and Protocols; find Sigma-Aldrich-H8161 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich

    A comparative high-throughput screening protocol to

    One of the challenges of developing antivirals is that most of these viruses are highly pathogenic and therefore require high biosafety-level containment. In this study, we have adopted a comparative high-throughput screening protocol to identify entry inhibitors for three enveloped viruses (Marburg virus, influenza virus H5N1, and Lassa virus) using a human immunodeficiency virus-based

    High-throughput screening (HTS) BMG LABTECH

    10-04-2019· High-throughput screening in drug discovery. The primary goal of HTS is to identify through compound library screenings, candidates that affect the target in the desired way, so called “hits” or “leads”. This is usually achieved employing liquid handling devices, robotics, plate readers as detectors and dedicated software for instrumentation control and data processing.

    Development of a Simple High-Throughput Screening

    01-12-2006· A high-throughput screening protocol has been developed for Mycobacterium tuberculosis glutamine synthetase by quantitative estimation of inorganic phosphate. The K m values determined at pH 6.8 are 22 mM for L-glutamic acid, 0.75 mM for NH 4 Cl, 3.25 mM for MgCl 2, and 2.5 mM for adenosine triphosphate.The K m value for glutamine is affected significantly by the increase in

    High-throughput Screening Steps · Small Molecule

    03-10-2017· High-throughput Screening Steps. Screening generally follows the following steps, which are included in recharge rates. This information is also available as a PDF: Guidelines for running small molecule screens, October 3, 2017.pdf. 3. 1. Consultation. Potential users are invited to discuss a screening idea with any member of the SMDC HTS group.

    Data Analysis Approaches in High Throughput Screening

    aspects of high throughput screening data interpretation. 2. Role of statistics in HT screening design 2.1. HT screening process A typical HT screening campaign can be divided into five major steps regardless of the as‐ say type and the assay read-out (Fig. 1).

    A Comparative High-Throughput Screening Protocol to

    02-07-2013· Experimental design of the comparative high-throughput screening protocol. (A) The comparative compound screen workflow. A549 cells are seeded into 384-well plates and infected by pseudovirions 24 h later in the presence of diverse compounds. After a 48-h incubation, the virus infection is detected by luciferase activity assay.

    A general protocol for creating high-throughput

    30-06-2009· A general approach to high-throughput screening of enantiomeric excess (ee) and concentration was developed by using indicator displacement assays (IDAs), and the protocol was then applied to the vicinal diol hydrobenzoin.The method involves the sequential utilization of what we define herein as screening, training, and analysis plates.

    A comparative high-throughput screening protocol to

    One of the challenges of developing antivirals is that most of these viruses are highly pathogenic and therefore require high biosafety-level containment. In this study, we have adopted a comparative high-throughput screening protocol to identify entry inhibitors for three enveloped viruses (Marburg virus, influenza virus H5N1, and Lassa virus) using a human immunodeficiency virus-based

    A modified high-throughput screening protocol to isolate

    19-02-2021· A modified high-throughput screening protocol to isolate bacteriophages from environmental samples Abdallah S. Abdelsattar 1,2, 1Rana Nofal1, Salsabil Makky,Amera El-Sayed1, Ayman El- Shibiny1,* 1 Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza, 12578, Egypt

    High Throughput Screening Methods and Protocols

    High Throughput Screening Methods and Protocols William P. Janzen Springer. Methods in Molecular Biology. Includes cutting-edge methods and protocols. Provides step-by-step detail essential for reproducible results. Contains key notes and implementation advice from the experts. see more benefits. Buy this book.

    Overview of High‐Throughput Screening Current

    01-03-2009· The section on assay methodologies now also covers enzyme complementation assays and methods for high-throughput screening of ion channel activities. Finally, a section on criteria for assay robustness is included discussing the Z′-factor, which is now a widely accepted criterion for evaluation and validation of high throughput screening assays.

    High Throughput Screening Methods and Protocols

    In High Throughput Screening, leading scientists and researchers expert in molecular discovery explain the diverse technologies and key techniques used in HTS and demonstrate how they can be applied generically. Writing to create precisely the introductory guidebook they wish had been available

    A protocol for high-throughput screening, analysis and

    24-03-2015· A protocol for high-throughput screening, analysis and validation of protein glycopatterns Abstract. In the past two decades, one of the main ways to investigate glycosylation was using lectins. Lectins are Introduction. Glycosylation is the most common form of co-translational and

    A cell-based high-throughput protocol to screen entry

    high-throughput screening (HTS) assay to identify entry inhibitors of three highly pathogenic viruses from Chinese Medicine extracts. We demonstrate that this protocol allows one to identify specific and “shared” entry inhibitors for different viruses with a greatly reduced number of false positives, alleviating a major problem in HTS.

    High-throughput stability screening for detergent

    17-07-2019· Our screening protocol allowed us to measure the stability and solubility of IMPs in a high-throughput manner and to determine the detergent or class of

    High-throughput screening and validation of antibodies

    22-02-2021· The antibodies were validated by high-throughput functional screening, and genome editing. Next, we explored the temporal dynamics of signaling in single cells.